Identification and physical mapping of induced translocation breakpoints involving chromosome 1R in rye
نویسندگان
چکیده
منابع مشابه
Agronomic Effect of Wheat-Rye Translocation Carrying Rye Chromatin (1R) From Different Sources
rived from Amigo have been recognized as the most favorable for yield (Lukaszewski, 1990; William and The confounding effect of wheat (Triticum aestivum L.) genetic Mujeeb-Kazi, 1993). T1BL·1RS resulted in higher grain background has been addressed as the major factor in inconsistent yield, kernel weight, and above-ground biomass (Carver agronomic performances of 1RS translocation. The objectiv...
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We report mapping of translocation breakpoints using a microarray. We used complex RNA to compare normal hexaploid wheat (17,000 Mb genome) to a ditelosomic stock missing the short arm of chromosome 1B (1BS) and wheat-rye translocations that replace portions of 1BS with rye 1RS. Transcripts detected by a probe set can come from all three Triticeae genomes in ABD hexaploid wheat, and sequences o...
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Balanced chromosome rearrangements (BCRs) can cause genetic diseases by disrupting or inactivating specific genes, and the characterization of breakpoints in disease-associated BCRs has been instrumental in the molecular elucidation of a wide variety of genetic disorders. However, mapping chromosome breakpoints using traditional methods, such as in situ hybridization with fluorescent dye-labele...
متن کاملMapping of balanced chromosome translocation breakpoints to the basepair level from microdissected chromosomes
The analysis of structural variants associated with specific phenotypic features is promising for the elucidation of the function of involved genes. There is, however, at present no approach allowing the rapid mapping of chromosomal translocation breakpoints to the basepair level from a single chromosome. Here we demonstrate that we have advanced both the microdissection and the subsequent unbi...
متن کاملMapping chromosome rearrangement breakpoints to the physical map of Caenorhabditis elegans by fluorescent in situ hybridization.
A scheme for rapidly mapping chromosome rearrangements relative to the physical map of Caenorhabditis elegans is described that is based on hybridization patterns of cloned DNA on meiotic nuclei, as visualized by fluorescent in situ hybridization. From the nearly complete physical map, DNA clones, in yeast artificial chromosomes (YACs), spanning the rearrangement breakpoint were selected. The p...
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ژورنال
عنوان ژورنال: Chromosome Research
سال: 2006
ISSN: 0967-3849,1573-6849
DOI: 10.1007/s10577-006-1089-7